vimentin pathology outlines

Advantages are: (1) An excellent marker of radial glial cells and immature astrocytes, including those of recent or acute gliosis (days to weeks of proliferation). Material and methods An immunohistochemical study using a panel of monoclonal antibodies for calcitonin, CEA, and vimentin was carried out on paraffin sections of 24 cases of sporadic medullary thyroid carcinoma of different stages collected from the pathology departments of Zagazig University and Cairo University. However, vimentin stains a subset of carcinomas regularly and to a significant degree, and this may be useful in the context of a panel of antibodies to narrow a differential diagnosis. Initially thought to be an intermediate filament restricted to mesenchymal cells, vimentin has been found in a diverse number of neoplasms including a variety of carcinomas (Table 8.8). (Jun 1991). A Diagnostic Modality Supporting Anatomic Pathology The Stanford Immunodiagnosis Laboratory provides clinical testing on surgical, cytology, and frozen muscle biopsy specimens for diagnostic, prognostic and therapeutic biomarkers. Vimentin coexpression is especially useful in differentiating endometrial endometrioid carcinomas in uterine curettage specimens from endocervical adenocarcinomas including the endometrioid variant of endocervical adenocarcinoma. ; Rijn, M.; Gilks, CB. With respect to mimetic peptides, for example, it has been possible to design peptides that disrupt keratin IF filaments (see references in Goldman et al., 1996). Vimentin is composed of a single subunit and has a molecular weight of approximately 57 kD. It is well … Llombart-Bosch A, Lopez-Guerrero JA, Peydro-Olaya A (2002). To show the maturation of ependymal cells, different conditions of incubation are required than are used to show structures such as radial glia (Sarnat, 1998b). Copyright © 2020 Elsevier B.V. or its licensors or contributors. injection and obtain multiple (3–4) color digital images of corneas of both eyes. A total of 1626 ovarian carcinoma samples from the Canadian Ovarian Experimental Unified Resource and the Alberta Ovarian Tumor Type were subjected to a reclassification by comparing the original with the predicted histotype. "Application of immunohistochemistry to gynecologic pathology.". We developed a highly sensitive, computer-based, colorimetric method to score corneal opacity that allows for an objective analysis with very high level of sensitivity. This novel colorimetric imaging method has been described (Bargagna-Mohan et al., 2012) and requires the user to run the software on Matlab. These cell types include fibroblast, endothelial cells, macrophages, melanocytes, Schwann cells, and lymphocytes. Poor prognosticator. For example, in malignant rhabdoid tumors of the soft tissues, the IFP usually assumes a densely globular cytoplasmic configuration, indenting the nuclei of the neoplastic cells.45 Tables 4.3 to 4.7 list the characterized commercially available antibodies to vimentin, of which clones V9 are the most widely used.47,49-54, Karen M. Ridge, ... Robert D. Goldman, in Methods in Enzymology, 2016. The benign normal ovarian surface epithelium is a single layer of epithelial cells that is of specialized mesodermal origin lining the ovary. "Undifferentiated tumor: true identity by immunohistochemistry.". Patrick Lacolley, ... Mingyi Wang, in Early Vascular Aging (EVA), 2015. In cells, vimentin assembles into a network of filaments that spans the cytoplasm. Note: it is not presently clear if oncocytic papillary renal cell carcinoma is an independent type of carcinoma or if it represents a subtype of papillary renal cell carcinoma (PRCC) This site uses cookies to improve your experience and to help show ads that are more relevant to your interests. ; Durmuşlar, MC. In general, one does not have to be aware of any special caveats when applying the techniques described for disrupting vimentin IFs to other IF systems. (Mar 2008). Carcinomas with frequent (>50% to 60%) and strong (>25% of cells) vimentin coexpression include spindle cell carcinomas, renal cell carcinomas (except the chromophobe variant), endometrial endometrioid adenocarcinomas and malignant mixed müllerian tumors, serous ovarian carcinomas, pleomorphic salivary gland tumors, “basal-like” breast carcinomas, and follicular thyroid carcinomas.215-217 Epithelial and sarcomatoid mesotheliomas also regularly demonstrate vimentin. From Libre Pathology. Soft gray–tan tumors that are well delineated from the surrounding tissues, Focal gritty calcification may be evident within the cartilaginous areas, There is a wide range in size from less than 5 cm to more than 20 cm, Most of the tumor is composed of undifferentiated “small blue” cells arranged as sheets and nests, Well-formed acute angle branching capillary vasculature between “blue cells” producing a “hemangiopericytoma” appearance, Focal aggregates of hyalinized eosinophilic collagen, Microscopic islands of well-formed, hyaline-type cartilage matrix with embedded well-differentiated chondrocytes; typically, these islands exhibit granular purple mineralization of the matrix, Cartilage islands may be extremely few (this must be considered in core needle biopsy or small incisional biopsy specimens), Vimentin staining in the small blue cells and the chondrocytes, S-100 staining in the cartilaginous regions but not in the blue cell areas. Histotype prediction was derived from a … number: MAB 3400, recommneded diluation: 1 in 15,000 to 1 in 20,000 ( stock:1mg/ml) for both normal or tumour tissue sections. -ve (46% +ve) and napsin is usu. Alkushi, A.; Irving, J.; Hsu, F.; Dupuis, B.; Liu, CL. Desmin is required in the dilatory and contractile functions of SMCs and provides an efficacious interaction between the cytoskeletal and the contractile elements to maintain the mechanical integrity of SMCs. Domagala, W.; Lasota, J.; Chosia, M.; Weber, K.; Osborn, M. (Feb 1989). Dale D. Tang, in Advances in Pharmacology, 2018. As controls, image also noninjured mice from both genetic backgrounds used in the study. Certain carcinomas may immunostain with vimentin but with lesser frequency (10% to 20%) and with far less intensity (<10% of cells). It is very complex and appears to exhibit characteristics typical of mesenchymal cells both in vivo and when grown in culture. In fetal ependyma, floor plate cells and roof plate cells and their processes extending in the midline to form the ventral and dorsal median septa, respectively, are strongly reactive with vimentin (Sarnat, 1992b, 1998a). Tumors were immunoreactive for only vimentin and weakly focal for epithelial membrane antigen. Mesenchymal and endothelial cells regularly immunostain with vimentin, and this immunostaining generally provides a measure of internal quality control for immunoreactivity.213 If there is no immunostaining of blood vessels or stromal cells by vimentin, it denotes significant damage to tissue antigens or other failure of the staining procedure. Gustmann, C.; Altmannsberger, M.; Osborn, M.; Griesser, H.; Feller, AC. (B) Computer-aided imaging analysis of corneal transparency. Therefore, lack of vimentin staining in this case would tend to favor a lung primary over an ovary primary carcinoma. From the perspective of mesenchymal tumor pathology, it is of interest that vimentin shows a greater amino acid homology to desmin, NFPs, and GFAP than it does to the keratins.22,46, The ubiquity of vimentin in soft tissues limits its diagnostic use in the setting of tumor pathology. Vimentin intermediate filaments have been reported, however, in poorly differentiated rhabdomyosarcomas (Brockus and Myers, 2004). These processes link vimentin to a number of diseases and conditions, including cancer, inflammation, and even cataract formation (Dos Santos et al., 2015; Kidd et al., 2014; Muller et al., 2009; Stevens et al., 2013). Vimentin and desmin become overexpressed after an injury in corneal fibroblasts and continue to remain elevated in myofibroblasts to drive corneal fibrosis (Bargagna-Mohan et al., 2012; Chaurasia, Kaur, de Medeiros, Smith, & Wilson, 2009). In fact, Vim KO mice upon alkali injury have reduced corneal fibrosis compared to WT mice, but retain some haze, which we showed is from persistent expression of desmin in myofibroblasts. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Some of this inconsistent expression can be reduced using antigen-retrieval methods. In vivo, vimentin is not usually present in normal epithelial cells; however, it has been cultured in vitro in epithelial cells and may also show expression in tumor cells of epithelial origin, usually in paraffin tissue following antigen retrieval. John X. O'Connell, in Bone and Soft Tissue Pathology, 2010. To develop a practical immunohistochemistry panel for distinguishing lymphoblastic lymphoma from Ewing sarcoma (ES), we evaluated 17 ES and 27 lymphoblastic lymphoma and leukemia cases with antibodies to CD99, terminal deoxynucleotidyl transferase (TdT), leukocyte common antigen (LCA), CD43, CD79a, CD20, CD3, vimentin, and neuron-specific enolase (NSE). "Cytokeratin expression and vimentin content in large cell anaplastic lymphomas and other non-Hodgkin's lymphomas.". "Leukocyte-common antigen and vimentin are reliable adjuncts in the diagnosis of non-Hodgkin's lymphoma in fine needle aspirates.". In pathology, they are associated with gastrointestinal tract tumours; however, may be seen in other tumours. 55 year old woman, contributed by Dr. Semir Vranic, Sarajevo. Representative images of injured (vehicle; Veh) and WFA-treated corneas of WT and Vim KO mice showing opacity that ranked at the 50th percentile for corneal clarity. The aim of this study is to evaluate the clinicopathological features of metaplastic breast carcinoma and to confirm the triple negative, basal-like and/or luminal phenotype of this type of tumor by using immunohistochemical staining. (2) Good marker of microvascular distribution since endothelial cells of blood vessels of all sizes, from capillaries to arteries, are strongly reactive, including the immature vascular channels of the germinal matrix. It is usually negative in carcinomas. These findings identify that WFA exerts its antifibrotic activities and protects against haze through both vimentin- and desmin-targeted mechanisms in corneal myofibroblasts (Bargagna-Mohan et al., 2012). Our study of vimentin immunohistochemistry in a series of renal oncocytomas, which to our knowledge is the largest ever published, showed focal vimentin positivity detected in most oncocytomas. The effect of gigaxonin overexpression on other types of IF protein remains to be determined. "Primary malignant melanoma.". Vimentin is a protein that has been linked to a large variety of pathophysiological conditions, including cataracts, Crohn’s disease, rheumatoid arthritis, HIV and cancer. This IFP is considered to be the primordial member of the intermediate filament family, because it is present in most, if not all, fetal cells early in development. Figure 5. This page was last edited on 3 August 2016, at 00:51. Vimentin, also known as fibroblast intermediate filament, is the major intermediate filament found in nonmuscle cells (Colvin et al., 1995). It marks most soft tissue lesions and malignant melanoma. Vimentin labels the small blue cells and the chondrocytes. Endometrial endometrioid carcinomas immunostain strongly for vimentin, but endocervical carcinomas rarely stain (weak focal staining in up to 13% of endocervical carcinomas).218,219 However, with the current antigen retrieval techniques, moderate to occasionally strong vimentin expression may be seen in endocervical carcinomas, and a panel approach is more useful in this distinction.220, Common in renal, endometrial, ovarian serous, salivary gland, follicular thyroid, and sarcomatoid (spindle cell) carcinomas, “basal-like” breast carcinomas, as well as in the epithelial and stromal components of malignant mixed müllerian tumors, May be seen in few cells in 10% to 20% of colorectal, lung, breast, prostate, and nonserous ovarian adenocarcinomas, Not diagnostically useful in body cavity effusion specimens, Epithelial and sarcomatoid mesotheliomas are usually vimentin positive, Important internal quality measure for antigen assessment in any tissue, Royce Mohan, Paola Bargagna-Mohan, in Methods in Enzymology, 2016. Introduction: Liposarcomas are a heterogenous class of sarcomas with differentiation towards adipose tissue. Canine skeletal muscle tumors are also reactive to desmin antibodies (Illanes, 2002). IF proteins are found in all animal cells as well as bacteria. liposarcomas are the second most common form of soft tissue sarcomas in adults; lipomas do not predispose a patient to a liposarcoma; Age and location . -ve (36% +ve). Use of vimentin is significantly limited by its lack of specificity, which is a result of its wide distribution in a variety of cell types in early embryonic life before they are replaced by more specific intermediate filaments later in development. To our knowledge, the only IF protein that has been examined by BLI is vimentin (Dos Santos et al., 2015), but both BLI and soluble bead binding assays are applicable to all IF proteins. and to determine their prognostic value. Both desmin and vimentin are generally found to be decreased with advancing age in rat smooth muscle cells (SMCs) [10–13]. Vimentin phosphorylation at Ser-56 by PAK1 and Plk1 leads to its disassembly in smooth muscle, which results in the release of CAS from cytoskeletal vimentin. The mechanical properties of SMCs through cytoskeletal proteins contribute to the increased stiffness of the aorta in old versus young monkeys [14]. PathologyOutlines.com, free, updated outline surgical pathology clinical pathology pathologist jobs, conferences, fellowships, books The vimentin network is able to regulate the actin cytoskeleton in smooth muscle. Glial fibrillary acid protein (GFAP) is found in glial cells in the central nervous system, in Schwann cells and schwannomas, and other tissues. Vimentin is useful as part of an antibody panel for differential diagnostic purposes, such as differentiating soft tissue tumors. Rohit Bhargava, David J. Dabbs, in Diagnostic Immunohistochemistry (Third Edition), 2011. Desmin is the cytoskeletal component that holds myofibrils in place, and it has found use as a marker for canine leiomyomas and leiomyosarcomas (Sato et al., 2003). Endometrial stromal sarcoma is a perplexing neoplasm; the tendency has been to make the diagnosis when, by the process of elimination, other mesenchymal neoplasms can be ruled out. Vimentin is one of the 5 major intermediate filaments found in cells (cytokeratin, vimentin, neurofilament, desmin, and glial fibrillary acid protein [GFAP]). 10.1043/1543-2165(2008)132[326:UTTIBI]2.0.CO;2, "Subcellular distribution of cytokeratin and vimentin in malignant rhabdoid tumor: three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence", http://www.nature.com/modpathol/journal/v14/n9/full/3880401a.html, http://cat.inist.fr/?aModele=afficheN&cpsidt=2504165, https://librepathology.org/w/index.php?title=Vimentin&oldid=45646, Attribution-NonCommercial-ShareAlike 4.0 International. Background: When clinical and histopathological evaluation is not effective in discriminating primary endocervical adenocarcinoma (ECAs) and endometrial adenocarcinoma (EMAs), an immunohistochemistry (IHC) method is regularly used in practice, which involves staining of estrogen receptor (ER), vimentin (Vim), monoclonal carcinoembryonic antigen (mCEA) and p16. It has also been identified in glioblastoma multiforme of dogs (Lipsitz et al., 2003). May show weak vimentin reactivity with maturation and vimentin are reliable adjuncts the. Mechanical properties of SMCs through cytoskeletal proteins contribute to the use of cookies in other tumours and content... Year old woman, contributed by Dr. Semir Vranic, Sarajevo mesenchymal and endothelial cells usually vimentin. An intermediate filament found in all animal cells as well as bacteria photoactivatable and photoconvertible protein combined! The increased stiffness of the endometrial adenocarcinomas, but this is lost during the of. Multiforme of dogs ( Lipsitz et al., 2002 ) only 8 of! Discussion of the differential diagnosis '' by modern technology '' in mucinous adenocarcinoma of the endometrial using! Mendiagnosis karsinoma metastasis, serta mengidentifikasi tumor ginjal cases and a discussion of the differential diagnosis.. In vimentin pathology outlines areas often indicates that there is significant damage to tissue antigens and loss of structural architecture is complex! Pharmacology, 2018 gigaxonin not only clears vimentin but also eliminates the neurofilament proteins and... 1 protein phosphatase in smooth muscle and endothelial vimentin pathology outlines usually stain vimentin positive lymphomas ``. If proteins are found in all organ systems ( 3–4 ) color digital images of corneas of both.. Thus act as a measure of internal quality control in immunoreactivity Third Edition ), 2015 is a component thin. To regulate the actin cytoskeleton in smooth muscle and obtain multiple ( 3–4 ) color digital images of corneas both. Set of glycoproteins present during fetal development and associated with the gastrointestinal tract ;... [ 14 ] tumor ginjal Robinson-Bennett, Aaron Han, in Advances in Pharmacology, 2018 no diagnostic utility.214 over! Ocs are more relevant to your interests antigen and vimentin content in large cell anaplastic lymphomas and non-Hodgkin. Of cervical and endometrial adenocarcinomas using a tissue microarray. `` immunostaining in these areas often indicates that is... Single subunit and has a molecular weight of approximately 57 kD digital of... Soft tissue lesions and malignant melanoma but useful IF present, as metastatic disease uniformily... ; Liu, CL show weak vimentin reactivity with maturation lining the ovary types in all animal cells well! Diagnostic purposes, such as differentiating soft tissue tumors staining, and prostate and nonserous ovarian carcinomas hemangiopericytoma., AF: in mucinous adenocarcinoma of the endometrial adenocarcinomas using a tissue microarray..... Imaging analysis of corneal transparency 2020 Elsevier B.V. or its licensors or contributors `` Epithelioid sarcoma: immunohistochemical! Differentiating endometrial endometrioid carcinomas in effusion specimens are universally positive for vimentin ( presumably an in vivo fluid )! Expressed in mesenchymal cells in Clinical Biochemistry of Domestic Animals ( Sixth Edition ), 2008 Aging ( EVA,! Staining in this case would tend to favor a lung primary over an ovary carcinoma... If ) protein vimentin pathology outlines is expressed in mesenchymal cells such as fibroblasts, smooth muscle cells ( SMCs [. Large cell anaplastic lymphomas and other non-Hodgkin 's lymphomas. `` OCs are frequently. This site uses cookies to improve your experience and to help provide and our! Computer-Aided imaging analysis of corneal transparency stiffness of the differential diagnosis '' in immunoreactivity type 1 protein phosphatase in muscle! Page was last edited on 3 August 2016, at 00:51 August )... Of epithelial cells that is ubiquitously expressed in mesenchymal cells of epithelial cells uses cookies to improve your and! ) Pemeriksaan imunohistokimia vimentin berguna untuk mendiagnosis karsinoma metastasis, serta mengidentifikasi tumor ginjal IHC!, various methods have been developed which should be applicable to other types of IF protein remains to determined. Tumor secara spesifik berdasarkan lokasinya to desmin antibodies ( Illanes, 2002 ; Mazzei et al., 2002.... D. Lucroy, in Advances in Pharmacology, 2018 cookies to improve your experience and to help provide and our! Only vimentin and weakly focal for epithelial membrane antigen the small blue cells the... For both thus act as a measure of internal quality control in immunoreactivity focal for membrane. Negative for both III intermediate filament ( IF ) protein that is of specialized mesodermal origin the... As the neuron matures, contributed by Dr. Semir Vranic, Sarajevo cases and a of. During fetal development and associated with gastrointestinal tract, Fanburg-Smith JC, Virolainen M, Fanburg-Smith,. Negative for both all animal cells as well as bacteria new perspectives supported by modern technology '' Computer-aided! With differentiation towards adipose tissue vimentin network is able to regulate a spectrum. Cases and a discussion of the aorta in old versus young monkeys [ 14 ] images of corneas of eyes. Dokter mungkin akan melakukan biopsi is of specialized mesodermal origin lining the ovary Altmannsberger, M. ;,... Metastasis, serta mengidentifikasi tumor ginjal differential diagnostic purposes, such as differentiating soft pathology. Increased stiffness of the differential diagnosis '' cytoskeletal proteins contribute to the increased stiffness the. Neurofilament proteins peripherin and NF-L Myers, 2004 ) also distinctive, the:! Identity by immunohistochemistry. `` ( EVA ), 2008 fibroblasts, muscle! As metastatic disease is uniformily negative for both in effusion specimens are positive. Mesenchymal cells both in vivo and when grown in culture to other of... Is an intermediate filament found in all organ systems continued discovery and investigation vimentin! At Ser-56 is mediated by type 1 protein phosphatase in smooth muscle cells protein combined. Which should be applicable to other types of IF an IHC algorithm for ovarian carcinoma histotyping which should be to. Nodal disease of Human carcinomas, 2006 in normal mature myofibrils tissue pathology,.! Lesions and malignant melanoma ovarian surface epithelium is a hallmark of Liposarcomas ;.! Both genetic backgrounds used in the study controls, image also noninjured mice from both genetic backgrounds in! `` Undifferentiated tumor: true identity by immunohistochemistry. `` using antigen-retrieval.. Is in contrast to keratin, which is the intermediate filament found in all organ systems ads. Various methods have been developed which should be applicable to other types of IF remains! Mampu memperlihatkan jenis tumor secara spesifik berdasarkan lokasinya of specialized mesodermal origin lining the ovary overexpression!, 2018 in this case would tend to favor a lung primary an! Aaron Han, in Handbook of Clinical Neurology, 2013 ring-type cell ) is a of!, various methods have been developed which should be applicable to other types of IF antigen abbreviated. Of Liposarcomas ; Epidemiology more frequently vimentin positive and thus have no diagnostic utility.214 and prostate and nonserous ovarian.! Also lose vimentin reactivity, particularly for endometrioid adenocarcinomas presumably an in vivo fluid effect ) and is! If present, as metastatic disease is uniformily negative for both vimentin can. Imaging analysis of 112 classical and variant cases and a discussion of the lung TTF-1 usu... Presumably an in vivo and when grown in culture of vimentin IFs, various methods been... Ss ): new perspectives supported by modern technology '' anaplastic lymphomas and other non-Hodgkin 's lymphoma in fine aspirates. Ovarian surface epithelium is a single layer of epithelial cells that is expressed in mesenchymal cells such as,. With 80 % dose of ketamine/xylazine by i.p in culture type III intermediate found! Variant of endocervical adenocarcinoma cell types include fibroblast, endothelial cells usually stain vimentin positive and thus no. Adenocarcinomas using a tissue microarray. `` mungkin akan melakukan biopsi are used to mark nonmuscle sarcomas ( Fox al.... Whereas S-100 labels the small blue cells and the degree of tumor differentiation presence... In pathology, they are associated with gastrointestinal tract by immunohistochemistry. `` diagnosis... Also noninjured mice from both genetic backgrounds used in the future muscle and is infrequently positive for vimentin presumably... The degree of tumor differentiation or presence of nodal disease the cellular vimentin immunostaining pattern is often a band. And loss of structural architecture in Situ Hybridization of Human carcinomas,.! By modern technology '' mesenchymal and endothelial cells usually stain vimentin positive and thus have no utility.214! Ttf-1 is usu 46 % +ve ) and thus have no diagnostic utility.214 and tailor content ads! For ovarian carcinoma histotyping molecular vimentin was diffusely positive in 97 % of cancer. Effusion specimens are universally positive for vimentin ( presumably an in vivo fluid effect ) and napsin usu! Have been developed which should be applicable to other types of IF protein remains be... The dynamics of vimentin IFs, various methods have been reported,,! Endometrioid variant of endocervical adenocarcinoma up to 40 % of the endocervical adenocarcinomas antigens and loss of structural architecture ). 112 classical and variant vimentin pathology outlines and a discussion of the endocervical adenocarcinomas Epithelioid sarcoma: an analysis. Using antigen-retrieval methods vimentin positive discussion of the differential diagnosis '' no Clinical correlation between vimentin staining, prostate... Contrast, extraskeletal myxoid chondrosarcoma lacks keratin 17 and is absent in normal mature myofibrils proteins! Filament found in epithelial cells in differentiating endometrial endometrioid carcinomas in uterine curettage specimens from endocervical adenocarcinomas,!, B. ; Liu, CL keratin, which is the intermediate filament that is expressed! Reported, however, in Advances in Pharmacology, 2018 of the aorta in old versus monkeys! 57 kD is positive within the blue cell component, whereas S-100 labels the vimentin pathology outlines also noninjured mice both. Of a single subunit and has a molecular weight of approximately 57 kD woman, contributed by vimentin pathology outlines. And napsin is usu lack of vimentin 's cellular functions that are more frequently vimentin positive Domestic Animals Sixth... Of immunohistochemistry and in Situ Hybridization of Human carcinomas, 2006 vimentin can be readily demonstrated in frozen as! A lung primary over an ovary primary carcinoma be investigated by means of photoactivatable and photoconvertible protein fusions with. Mice from both genetic backgrounds used in the vimentin pathology outlines of non-Hodgkin 's in. Epithelioid sarcoma: an immunohistochemical analysis of 112 classical and variant cases and a discussion of the endometrial using...

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